Our tool can be used to predict both antigen and neoantigen.

1. For antigen, 11297 experimental IEDB epitopes were used for training involved nine main HLA. We recommend using peptide of length 8-12 but not less than 8. N-terminal, position 2, C-terminal were anchored by default.

2. Neoantigen model need to provide additional wild sequence to extract mutation associated characteristics.

3. Predictive value greater than 0.5 is considered as immunogenicity. you can use example to test it.

Notes: please make sure that HLA matchs your peptide. In mismatched HLA, % rank value difference may strongly influence the accuracy of the results.